I am using ethanol to dissolve curcumin and then followed by dissolving again in PBS but it doesn't dissolve properly.
When using DMSO 10% to dissolve curcumin it doesn't dissolve properly too.
Still some powder particle precipitate in the tube.
Need your advice...
Methanol can be used. Check the link below for a paper if it is useful...
http://www.interscience.in/IJPPT_Vol1Iss2/81-84.pdf
I think the PBS step is where the problem lies. Curcuminoids are lipid soluble. Their solubility in aqueous phase is vanishingly small.
It depends on the methods and analyzes that apply. I think you could try with methanol.
try to use on of systems
EtOAc–HCOOH–HOAc–H2O, 30:1.2:0.8:8
EtOAc–Et2O–dioxane–HCOOH–H2O, 30:50:15:3:2
EtOAc–EtCOMe–HCOOH–H2O, 60:35:3:2
Dear All, thank you so much for your suggestion, in many ways using EtO I found non homogeneous suspension. But after many times trying to use DMSO as solvent it was showing good homogeneous suspension. But the quantity of DMSO quite high.
So, is there any adverse effects of DMSO if it is applied for solvent, which the solution will be applied in food treatment?
I may be too late but you could solubilize curcumin in many available lipid based solubilizers that are approved for human use.
Hi Professor In this process, have you examined the effect of temperature?
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