I have sequences of 16s bacterial specific polymerase chain reaction from bollworm so how to do analysis what type of study can be performed please explain
What do you want to do? And what was your aim to sequence the DNA samples?
But, you can only do similarity based analysis to get what tose sequences are and a phylogenetic inference to see the placement of those sequences with respect to each other.
Sequence information can be utilized in a variety of ways including similarity analysis (by performing sequence alignments) to determine if there are any similarities or differences in these sequences. You can further BLAST your sequences in the NCBI database to try and identify any earlier sequenced data that is similar to your sequence data which can give you an indication of the species or genus to which your sequences belong. In addition, you can perform phylogenetic analysis on those sequences using bioinformatics software like MEGA.