Hi
I hope someone can help me out. I have no knowledge of RAMAN or FTIR but have to do look at some data from these methods on some shell samples.
I have the data, I have used Spectrogryph to get the spectrum on each sample. I have the peak numbers and I can calculate the area under the peaks. BUT:
- Do I just pick two of the strongest peak and calculate the area, divide them and that is the peak ratio? and then do I just do the same to the other samples using the same start and stop points for calculating the areas as for the first sample for comparison?
Or do I use different start and stop points for each sample?
And can I use this method for both Raman and FTIR?
Hope someone can help out with a "layman" explanation. Thank you!
Rikke
you can calculated FTIR peaks and comparing between the different samples using the origin or OMNIC program.
Your question can't really be answered without knowing more about the data. If you just compare the peak area of the strongest respective peaks in two different samples, there may or may not be any information at all. You would have to compare the same peaks, if present, to compare the amount of bond that causes the peak. However, if the samples differ too much, there might be overlap between different peaks leasing to a false ratio.
Hi Adrian.
Thank you for the answer.
The samples does not really differ that much. They are very much the same.
Rikke
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