hello :)
I want to know if there's any way that I can separate the effect of superoxide radicals on cell death from immature senescence in Arabidopsis cells/leaves. I need to design an experiment which can possibly show that my mutant plants die faster because of early activation of senescence. By the way I have already checked the expression of many senescence associated genes in my mutant plants and they are up-regulated.
So i want to categorize my question into :
1) is there any biochemical method which can separate these two?
2) is senescence associated with a specific phenotype or sub-cellular change which i can look for further and prove my point? (for instance a change in the shape of vacuole)
Thanks in advance.
Hi Shirin,
There is a paper on animal cells talking about the staining of the beta-galactosidase to investigate the senescence of cells.
Maybe it could be relevant to your field too.
Good luck
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