I have been reading various papers on this but every paper is referring to a new distinct chemical combination, Please tell me if there is a standardised set of chemicals and growth factors that we can use for this conversion.
Hello
You can used two different protocols for neuronal differentiation of hUCB-MSCs. The first concect of four-step induction protocol consisting of exposure to a series of growth factors that push the MSCs towards a neuronal lineage . Briefly, the cells exposed for 3 days to Step-1 medium consisting of IMDM supplemented with FGF-2 (5 ng/ml), retinoic acid (0.5 μM) and 1 mM 2-mercaptoethanol, followed by 3 days in Step-2 medium consisting of IMDM supplemented with 1 mM cAMP and 100 μM AsA. The cells were further exposed to Step-3 medium consisting of IMDM supplemented with 10 μM hydrocortisone and 1 mM cAMP, followed again by 3 days in Step-4 medium consisting of IMDM supplemented with 20 ng/ml αFGF, 10 ng/ml Shh, 10 ng/ml brain-derived neurotrophic factor, 10 ng/ml nerve growth factor, 25 ng/ml vitronectin, 100 μM AsA, 0.1 mM 3-isobutyl-1-methylxanthine, 10 μM forskolin and 20 nM phorbol myristate acetate. For the second protocol involving direct neuronal differentiation, used normal neuronal differentiation medium containing neurobasal medium supplemented with FGF-2 (10 ng/ml) and 1% FBS for 5 days.
Check on articles here
Good Luck
Thanks a lot, the second protocol looks easier to me as compared to the first one.
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