I am working on wilt complex of chickpea, wilt caused by F. oxysporum f.sp. ciceri and black root rot caused by F. solani, so how can I identify particular Fusarium species?
Characters of F.oxysporum on CLA.
Macroconidia are formed in pale orange, usually abundent, sporodochia. the macroconidia are short to midium in length, falcate to almost straight, thin walled and usually 3-septate. The apical cell is short and is slightly hooked in some isolate. The basal cell is notched or foot-shaped. Macroconidia are formed from monophialides on branched conidiophore in sporodochia and to a lesser extent from monophialides on hyphae. microconidia usually are 0-septate , may be oval, elliptical or reniform (kidney-shaped), and are formed a bundantly in false heads on short monophialides .Chlamydospores are formed a bundantly in hyphae on the agar surface by most isolates .
Characters of F.solani on CLA:-
Macroconidia are relatively wide, wide straight to slightly curved, 3-7 septate with rounded ends and found abundantly in cream and less frequently in blue or green sporodochia. oval ,ellipsoidal or reniform ,0-or 1-septate microconidia are formed in round false heads on relatively long monophialides. some isolate are homothallic and my produce red or orange perithecia. Chlamydospores are produced abundantly in pairs in hyphae an in the agar.
Leslie and Summerell (2006).The Fusarium Laboratory Manual.
Best Regard
Hi, This is very helpful book for Fusarium spp taxonomy.
Book The Fusarium Laboratory Manual
The answer given by Mohammed Fayyadh is enough. Long monophialides means more than one, and rather even more than two lenghts of macrokonidium.
Comparing macrokonidia of F. oxysporum and F. solani - the second one has rather big and stout konidia. They are easy to distinguish also on PDA medium.
Several characteristics could be used to distinguish between the two species. Fusarium oxysporum produces a lilac to purple coloration of the stroma on PDA, and when viewed from the underside, exhibits dark purple streaks radiating from the center of the colony; F. solani produces cream to dark blue, but never purple, coloration of the stroma. F. solani produced pionnotes, whereas F. oxysporum does not. As far as microscopy goes spore shape as described by Buxton and Perry (1959) is very distinctive. Fusarium solani spores were broader and less pointed or not at all.
I recommend you read the leslie manual, you can feverything
https://www.researchgate.net/publication/321385629_The_Fusarium_Laboratory_Manual
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