Can anyone help me to remove a hydrophobic peptide from C18 column?

I have injected a hydrophobic peptide into analytical C18 column and i have tried to cleaning the column applying a lot of protocols using acetonitrile, methanol, isopropanol, but in the blank...

05 January 2021 3,305 3 View

Could you help with a doubt regarding UDF velocity inlet profile with multiphase mixture (Ansys Fluent)?

We are two undergraduate students seeking for help!!! We are trying to simulate a laminar flux through a 3D pipe (25 cm long, 2 cm of diameter) consisting of a multiphase flux (air and water). We...

23 November 2020 3,918 3 View

What is the role of Digital Services in Crisis, Disaster, and Emergency Situations?

06 January 2020 7,241 4 View

How to dissolve Ibuprofen?

I need to obtain IBU aqueous solution. Some papers report that they dissolve it in water/methanol (90/10). But it did not work. What to use to dissolve it? Some buffer or NaOH? Or maybe some salt....

05 May 2019 6,575 1 View

How come conductivity increased at very low concentrations of CuSO4 after sorption of activated carbon?

I was investigating the sorption of activated carbon on different concentrations of copper(II)sulfate and came across rather unexpected results where the conductivity increased at 0,00001 , 0,0001...

24 November 2018 6,855 3 View

What is a good cycling method for 27F and 519R primers? Tm is 56 and 66, can they be used as a pair?

I'm trying to generate a smaller fragment of 16S from a water sample, so it can be NGS amplicon sequenced. The limit of that service is about 500bp.

14 March 2018 8,032 2 View

Can I use aneuploidy for crossing?

Dear Collegues, we are going to make segregating population of tea plant (camellia sinensis) for genetic research. we need 200 progenies. Can we use aneuploid cultivar as one of the parents to...

19 October 2017 8,451 3 View

Protocol of fungal dna extraction from plant leaf tissue?

Dear collegues, Can we use protocols of DNA extraction from plant tissues (from leaves) to extract DNA of fungal pathogen which lives inside of tissue? If yes, will the quantity of the pathogen's...

11 April 2017 8,448 31 View

If I have low bootstrap values in a ML tree, does it mean that there is no separation between different genera?

I have a maximum likelihood tree (18S and COI) for a nematode family which contains 4 different genera. The bootstrap values were very low and all the 4 genera are intermingled in both trees with...

27 June 2016 593 5 View

Why does the PCR not work occasionally?

Dear collegues! we extracted RNA from apple peel. We need clean RNA without DNA contamination, so we check our RNA samples by PCR. Samples are fresh. and reagents are the same. BUT one time PCR...

24 March 2015 4,855 12 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

What type of SPSS analyses should I use for my Dissertation?

I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...

22 February 2021 1,351 4 View