what is the best method to determine the dominant orientation of collagen fibers?
i preformed FFT and shited the frequencies , what should be my next step ?
Have a look at https://wArticle Rapid Quantification of 3D Collagen Fiber Alignment and Fibe...
ww.science.gov/topicpages/c/collagen+fiber+orientation.html and
If you use a FFT Transform. You need to detect two peaks, However in your FFT images obtained from Collagen Fiber is very complex.
I recommend use the Hough Transform to detect lines and after filtering using the bigger lines. Matlab and python has implemented this algorithm.
Rapid Quantification of 3D Collagen Fiber Alignment and Fiber Intersection Correlations with High Sensitivity.
Article Rapid Quantification of 3D Collagen Fiber Alignment and Fibe...
In addition to the previous answers, maybe this can help you out:
Article Fitting-free algorithm for efficient quantification of colla...
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