please explain in detail about the method for the estimation of aldehyde groups in oxidized polysaccharide. The oxidized polysaccharide forms slurry when dissolved in any solvent.
Hello Tariq,
Oxime conversion method and UV-Vis analytical technique using t-butyl carbazate and trinitrobenzenesulfonic have commonly used.
Please see more details on page 340 of this paper
https://doi.org/10.1080/07328303.2015.1068793
Also there is a similar Q/A available on R/G
https://www.researchgate.net/post/Help_required_regarding_how_to_determine_aldehyde_content
Regards,
Amin
Try UV-Vis technique using 2,4-dinitrophenylhydrazine (DNPH), its widely used to calculate the aldehyde content in oxidized polymer:
*Run UV spectra for oxidized polysaccharide
*Then Run it for hydrazone resulted from DNPH+polysaccharide reaction (it will be shifted to different wavelength)
*by applying well know Beer's law (A=ebC) you can get the amount of aldehyde, e can be measured from the calibration curve for slandered DNPH, b is the cell length and A is the absorbance, C the concentration mol/l
Thanx Dear Amin Shavandi and Fateh Eltaboni for your suggestions.
For UV-Vis technique the oxidised polymer should be first completely soluble but the problem is the product makes suspension when dissolved in any solvent. i also did the oxime conversion by schiff base reaction with hydroxylamine hydrochloride ,the results obtained were not satisfactory.
Hi Tariq Mir
Try then solid state IR spectroscopy by quantifying C=O peaks
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