How to determine cellulose, Hemi-cellulose, Lignin Content of natural fiber?...What type of chemical treatment should done to determine these contents....Please provide the details which you find very useful.
Dear Linto,
There is no easy answer, but perhaps an XRD measurement might help towards quantitative and qualitative analysis of the components in your fiber. You could use either the traditional XRD or EDAX (SEM), depending upon the amount of material you have.
I hope it helps.
Thank you...Aditya chauchan and Ankita sharma ...But i need the chemical methods which determine the chemical compositions content/ percentage present in natural fibre..Not XRD or FTIR....EDAX(SEM) for the elements...such as carbon oxygen etc.
XRD or EDAX won't give any results regarding cellulose , hemicellulose percentage content etc.
Another thing People already did the works...Easy methods are there...But they won't like to share..
And Ankitha sharma..U r suggestions is good... i agreed...FTIR gives chemical composition by identifying the chemical groups present..But it won't gives how much percentage of content present in the fibre...Even in your paper on sisal fibre composites..what is the reason u did FTIR spectrometry....How it helps in composites..
You can search for the Van Soest method to determine the biochemical composition (this is a very common method). In some papers from A. Bourmaud or C. Baley for instance, you can find some informations about how they did to measure the cellulose, hemicellulose, lignin, pection contents... Carbohydrate Polymers and Industrial Crops and products are two journals in which you will find several papers dealing with that. Besides, you can also search for books about wood and biomass chemistry (some are very old)...
Actually, WAXD can give you a cellulose crystallinity index which is directly correlated to the cellulose content. As cellulose is the only crystalline component in natural fibres, you can determine the amount of amorphous components through this method. FTIR can be useful for a comparison but not for absolute values, I think.
There is a Chinese standard, GB5889-86: Method of quantitative analysis of ramie chemical component. This can be applied for other natural fibres to determine amount of cellulose, hemicellulose, lignin etc. I read many papers where the authors claimed to use that standard. Unfortunately it is written in Chinese. You can search if you get any English version. :)
There are severla methods to determine the composition: Tappi, Din, ASTM...
You can find many free published work in google to determine that
i found several methods to measure the chemistry composition by using NDF (neutral detergent fibre) and ADF (Acid detergent fibre).
Dear Sir,
Please kindly go through this paper.
https://www.sciencedirect.com/science/article/pii/S0144861718314152
you can easily determine the composition through TAPPI methods... or else u can do some chemical reactions for that, easiest way to analyse, refer this article..
https://asmedigitalcollection.asme.org/energyresources/article/doi/10.1115/1.4032729/444232/Physicochemical-Characterization-and-Pyrolysis
Determination of Lignin content: https://scialert.net/fulltextmobile/?doi=pjbs.2002.521.522
Determination of Cellulose content: Article Quantitative Determination of Cellulose in Raw Cotton Fiber....
There are chemical methods by which the chemical composition of the natural fibers can be determined. For example TAAPI procedure in which the individual components (alpha cellulose, hemicellulose and lignin) are separated and determined quantitatively. There are several research papers on the properties of natural fibers (such as Hildegardia populifolia uniaxial natural fabric, Tamarind Fruit fibers, Borassus fruit fibers, Ridge guard 3 dimensional fabrics etc) available in the literature which describe these procedures.
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