Hello, I am still new to this, if I may ask, how do you do the dilution for gallic acid?
Just now I have 100ml of gallic acid stock solution from dissolving 0.500mg of Gallic acid + 10ml Methanol and diluted to volume with Distilled water.
I need to make 10, 40, 80, 140, and 200 microgram/millilitre for the standard curve but I do not have any idea how to do it, thank you..
To prepare a standard calibration curve of Gallic acid it is better to take different concentrations of Gallic acid (10 to 100 μg/ml) in methanol, it may be 10, 20, 30, 40, 50 μg/ml and so on.
Good luck!
Good day. To get started, you need to know the gallic acid concentration of your stock solution. Knowing the concentration, you can make a standard curve using the formula C1V1 = C2V2. Example: Your stock solution has 100 ug / mL of gallic acid. To make a 10 ug / mL standard in 100 mL, you will need 10 mL of the standard. (100 ug / mL x 100 mL) / 100 ug / mL = 10 mL 10 mL gallic acid + 90 mL of water = a 10 ppm standard in 100 mL of solution.
Please look at the following below links which may help you in your analysis:
https://aip.scitation.org/doi/pdf/10.1063/5.0002657
https://rjp.nipne.ro/2016_61_7-8/RomJPhys.61.p1255.pdf
https://iopscience.iop.org/article/10.1088/1755-1315/807/2/022067/pdf
Thanks
Thank you everyone for answering, Sir Juan Alemán I have a question, if 10 ml of standard stock solution needed to make 10 ug / mL of gallic acid, does that means I need 120 mL of stock solution to make 120 ug / mL gallic acid for the calibration curve?
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts