Hello Haslawati, I'm khairul from Research Institute for Fish Breeding, Indonesia. I'm glad to know you. I think your project research same with in my institution. We are studying Koi Herpesvirus (KHV) too. Maybe we can share information about it next time. Oh ya for your question, Firstly i think you can sequencing the KHV DNA that you have, and you can analyze/align it into GeneBank by BLAST software.

I am actually helping my colleague in Dept of Fisheries (Biosecurity Division). Since my interest is more in fish taxonomy (molecular and morphology) and evolution stuff, I just wondering whether the same method (sequence analysis) is also applicable to understand the mutation process in KHV sequences? Thanks anyway for your interest, indeed we could share some result and discussion.

Of course, i think you can analyze any organism mutation (especially in DNA level) by sequence analysis. By the way, what is research program about KHV in your institution?

From what I know, this is may be the first report (there were reported earlier from fish consumption to Singapore) on KHV surveillance in Peninsular Malaysia. I will try to analyse the sequence as suggested. We used RT-PCR and direct sequencing in this study. To be frank, I am new to virus study, but I have got some training in PCR, DNA sequencing analysis and phylogenetics.

Ok, i think your experiences by training are good and enough to study mutation in any organism (DNA level especially). Do you know what KHV strain that was found in Peninsular Malaysia?. You know, KHV strain in Indonesia is similar (99%) with in USA and Israel (Acc Number in GeneBank: AP008984 (Indonesia), DQ657948 (USA), DQ177346 (Israel)).