How to design primers for circular RNAs for Validation of RNA sequence data?

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Ibrar Muhammad Khan

Dear Sahlu!

Try the back-splicing method to design a primer for circ-RNA. This method is specifically used for circ-RNA primer designing...

Good luck

Liora Yesharim

Hi Bahlibi Weldegebriall Sahlu,

I found the circPrimer software to be extremely useful for this purpose. It allows you to visualize circRNAs, obtain their sequences, and design divergent primers for your specific circRNA of interest. You can find more information about this software in the linked article:Article CircPrimer: A software for annotating circRNAs and determini...