How to design Arbitrary Degenerate primers for TAIL PCR?

Muneeswari Alias Surya S @Muneeswari-Surya-S

26 November 2019 0 3K Report

I am working with pCAMBIA vector for plant gene expression. Can anyone tell how to design arbitrary degenerate primers. What are the bases used for AD primers? (bases like N, R, Y, S and W). I could not find about designing part. I am getting only the designed AD primers. Could anyone help me with this

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