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I am working with pCAMBIA vector for plant gene expression. Can anyone tell how to design arbitrary degenerate primers. What are the bases used for AD primers? (bases like N, R, Y, S and W). I...
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Recently, I have done PCR for my gene of interest. in there, I got band size of my gene of interest and also in negative control (Water). I get the same band size of my gene of interest in...
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