How can writing tasks be designed or redesigned to accommodate the diversity in a multi -level ESL classroom?

Designing writing tasks in level- playing conditions of diverse groups in ESL settings calls for structural adjustment with the established notions of task design features. Yet , the culture of...

21 October 2020 9,469 6 View

How to Convert large fastq file to fasta?

Hello, I'm new to bioinformatics, my NGS dataset consists of 50 FASTQ files and each file is of size 10-15 GB.. I need to convert these FASTQ files to FASTA files. P.S. I am using google colab I...

02 July 2020 6,224 2 View

RNA Quantity & Quality extracted from Escherichia coli in M9 supplemented with different sugars?

I am trying to extract RNA from Escherichia coli grown in M9 minimal media supplemented with different sugars, however, every time the RNA extract ranges from 25 to 50 ng/ul. I am using the Qiagen...

23 October 2018 992 2 View

Is there an online source to know the equivalence of each OD to cfu/ml for Escherichia coli?

Is it the same for all media or differs from LB to minimal media? Doesn't OD correlate to cell mass? To be sure should i do a viable count for the growth of E.coli in specific media?

20 October 2018 2,588 3 View

Best Gel documentation system ?

02 April 2018 6,383 4 View

Electroporation of cripr cas9 by gene pulser?

Kindly I need to do transfection for canecr cell lines by Crispr cas 9 from santa cruiz (downregulation and overexpression) , I wonder if cuvette 0.2 will work with it as I don't have 0.4 and the...

28 March 2018 3,398 2 View

Size of electroporator cuvette size for mammalian transfection ?

I am trying to transfect SNU449 cell line by GFP plasmids using genepulserX Bio rad electroporator. I wonder if I can use .4 cm cuvette and voltage needed ! and what is the cell size of SNU449 !

21 March 2018 7,864 3 View

No title

I am trying to do transfection of SNU 449 hepatocellular carcinoma cells by GFP plasmids using Bio Rad micropulser electroporator ! according to its protocol it nables safe and reproducible...

13 March 2018 8,271 3 View

How to combine Response spectrum analysis results with static structural results in ansys workbench ?

I am working on load combination effects on structure and i need to combine the results of response spectrum analysis with static structural ... How can i achieve the desired results ?

10 October 2016 3,322 4 View

Please, I would like to ask about the calculation of the annealing temperature of that primer?

07 August 2016 9,183 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

How can we improve AI in detecting fraudulent activities on digital payments?

I am conducting a study on the effectiveness of AI towards detecting and averting digital payment fraud. What are the popular AI techniques being adopted in mitigating digital fraud and how could...

21 February 2021 3,888 4 View

Are we using now IT technology effectively?

I discover now two my papers, which are more then 30 years old and which are describing something, what was done in years 1985 -...

21 February 2021 5,008 3 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View

Nuances in the digital twin concept. Opinions?

Hello everyone, I am currently working on the state of the art of the digital twin concept, and after seeing the categorization made by Kritzinger, W. et al. (2018) [Digital Twin in...

14 February 2021 2,107 3 View