hello, friends, anyone can explain to me if the insect mortality reached 100% in treated treatment, and we found 10% mortality in control .how we can justify corrected mortality? what about mortality in control treatment? because according to Abbott, W. S, 1925, formula: 1-nt/nc X100 where, n= insect population, t= treatment, c= control. when we use the Abbot formula the answer is 100% mortality. I am confused how to justify 10% insect mortality which found in control, is this formula correct? Let suppose I have test 20 insect with insecticide and got 100% mortality after few days. But in control treatment I found 18 insects, 2 were dead. 10% mortality was observed. how to define corrected mortality?
questionable not justified really....
http://www.ehabsoft.com/ldpline/onlinecontrol.htm
try other formulas at above link...
Hello Ghulam, I think Abbott correction work best for your data. You can use this Abbott formula- Corrected mortality = (treated mortality %- mean control mortality %) / (1-mean control mortality %).
thanks, friends, I have justified above formula, the formula was correct.
Corrected mortality must be less than 100% because accepted observed mortality should be less than 100%.Salam best regards
Abbotts correction (corrected %mortality= 1-(t2 x c1 / t1 x c2)*100 where t1 is the initial population in treatment; t2 is final population in treatment; c1 is initial population in control and c2 is final population in control))
You need to make screening test.You need to have the highest concentration when observed mortality is less than 100 % and the lowest concentration when observed mortality more than 20 % or more than control .Salam and best wishes.
Agreed with Dr Ronilson to decrease the concentration to have mortality less than 100% i.e to do screening test to determine the highest concentration which able to kill less than 100 %and for the lowest concentration which able to kill about 20% but more than the control .The control should be around 5% mortality .You need to add at least two concentrations located between the obtained highest and the lowest concentrations .Salam and best wishes .
Hi
I agree with Dr Al Antary
But a note, My Opinion, first, obtained 20 to 80% concentrations of insect Mortality then concentrations three of between (20 to 80%) calculated by AntiLog formula, also we accepts approximately 20% insect mortality in control. After doing tests, we can determine corrected mortality with used of Abbotts correction in lab condition.
The Best Regard
Samaneh Mahmoudvand
We are about in agreement Dr Samaneh, but differed in details.Thank you for your opinion .Best wishes .
Dear Dr. Ghulam
Please read the attached files ( Statistical Methods in BioefficicacyTrials and Probit Analysis)
Best wishes
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