How to determinate phosphatidylcholine content in cow milk?

I would like to determinate the phosphatidylcholine content in cow milk. We only have a Shimadzu Prominence HPLC (High Performance Liquid Chromatography) with a fluorescent detector for the...

18 January 2021 8,370 6 View

The chemical and microbiological properties of camel milk do have a significant effect on shelf life of camel milk powder compared to cow milk powder?

I need to know effect of heat treatments that are made when making camel milk powder on chemical , microbiological properties and shelf life of camel milk powder compared to cow milk powder. Thanks

11 January 2021 7,097 12 View

Why the taste of foods(item) changes with the sources of fuel we used(bio fuel,wood,dried dung) reality or meth ?

As my experience and many consumer experience they feel the different taste of the same item served cooked in a different source, is it real or not, what is the reason behind this.

24 December 2020 6,476 3 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

How should I store the milk sample in RNAlater?

I want to extract RNA from human breast milk. How should I store the milk sample in RNAlater? And after RNA extraction how can I store the RNA?

01 December 2020 7,183 4 View

Which buffer should I use to aliquot antibody in?

for western blot analysis the buffer in which the antibody already is, written in the datasheet, or should I use already the bsa or milk solution that I use for final diluition?

17 November 2020 4,804 3 View

What is the suitable temperature to store Vit A, E, D sample extract extracted from milk powder?

I run the sample preparation using AOAC Vit A,E,D method, that require saponification, concentration , drying using nitrogen gas, reconstitute with methanol. Since the HPLC method development is...

23 October 2020 6,430 4 View

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08 October 2020 2,907 3 View

What's typical DNA yield and quality of DNeasy Powered Food Kit?

I spiked in 4-8 log CFU/ml of Lactococcus lactis into milk, and use DNeasy Powered Food Kit to extract DNA from it. Nanodrop 2000 was used to determine the values. The yield and quality are weird:...

04 October 2020 6,211 4 View

How can we explain the decrease in taste in a patient with desminopathy (myofibrillar myopathy)?

A patient with hereditary desminopathy (mutation Thr341Pro DES in a heterozygous state) with disease progression has a significant decrease in taste. How can this fact be explained?

15 September 2020 3,742 4 View