How to deal with low amount of Chip-DNA for qPCR validation?

08 March 2020 1 3K Report

I need to perform Chip-qPCR as a validation step. Trouble is i have low amount of ChIP-DNA with me for qPCR. Is there any way I can solve this issue.

Thanks in Advance.

Hendry Susila

Hi,

The low amount is not a problem. The concentration of my ChiPped DNA (with TF) usually around 2-5ng/ul. Just continue the qPCR step, I usually use 1ul template for 10ul qPCR reaction.

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