The freezing stock was split, and after 3 days floating cells started appearing, so I changed the media by aspirating the old media and adding fresh without trypsinizing. The next day cells appeared dead/unhealthy and were floating so I trypsinized and seeded them in 6 well plate. None of them grew. Is there anything specific I should have done to prevent this? Do A172 have specialized culture conditions that should be kept in mind?
Thanks in advance
-Aditi
Ham's F12 medium (Invitrogen) and Dulbecco's modified Eagle's medium (DMEM, Invitrogen) were combined to create the culture medium for the cell line A172. L-glutamine and 10% foetal bovine serum were added as supplements (FBS; Sigma-Aldrich). The cells were cultured at 37 °C in a humidified incubator with 5% CO2.
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