Dear all,
I would like to cultivate Human Dermal Fibroblasts: HDF, neonatal (106-5n, Sigma Aldrich).
This type of cells are cultivated in the Fibroblast Growth Medium (116-500) according to the protocol. In addition to the classic Calf serum and antibiotic, is it necessary to use growth factor and insulin, as with other neonatal cells?
Or is there any alternative for it?
Thank you!