I have problem with my Chromobacterium violaceum failed to produce violet pigmen. I cultivate my Chromobacterium on luria broth with Tryptophane but nothing happen.
Riveros et al. (1989) Effect of growth conditions on production of violacein by Chromobacterium violaceum (BB-78 strain). Braz J Med Biol Res. 1989;22(5):569-77.
https://www.ncbi.nlm.nih.gov/pubmed/2620169
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". . . Methionine was the only absolute requirement for growth . . . The highest yields of violacein were obtained by incubation in liquid medium and in the absence of light at pH 7.0 and 28 degrees C for 24 h."