I cannot detect Parp protein after total protein isolation?

I cannot detect Parp protein after total protein isolation Hello everyone; I cannot detect the Parp protein after total protein isolation. I can see my cells die morphologically after a novel...

18 July 2019 2,593 2 View

At what stage should I use the DNAse I reaction protocol?

Protocol content (NEB DNAse I reaction Protocol) : RNA: 10ug DNAse reaction buffer 1X 10ul DNAse (Rnase free) 1ul Nuclease free water 100 ul My question is; if I use this protocol after isolating...

11 January 2019 1,249 3 View

GNSS Carrier phase Single difference residuals?

Dear all if I have the SD carrier-phase measurements how can I compute the SD residuals? Do i need to calculate the SD residuals before fixing the integer ambiguity?

10 April 2018 7,001 2 View

Is there any clear procedure how to measure the zeta potential of bacteria (in suspension not freeze dried)?

Thank you in advance Best Cigdem

18 January 2015 6,732 2 View

When is it a good time to consider relative risk reduction and when is it a good time for attributable risk reduction?

The above question is a prevalence question. If the prevalence of disease is high (and assuming that the disease is serious), we would consider ARR (=Risk in unexposed - Risk in treated ) versus...

06 May 2014 9,720 2 View

Is GC content important when designing oligonucleotides for transfection?

Is GC content important when designing oligonucleotides for transfection? Is this a problem for oligonucleotide-based transfection, where the dna region I will design oligonucleotide is very rich...

01 January 1970 988 3 View

How to release matter from genipin crosslinked polymer?

01 January 1970 4,805 4 View

Do you need specially designed vectors (such as addgene) for Crispr / Cas9 ?

Do you need specially designed vectors for Crispr / Cas9 ? If so, why? Can't I clone the gene into the restriction site of a vector I have?

01 January 1970 6,370 2 View

What do you think about the state of the cell?

HEK-293T is becoming this for a while after being in culture. (20X magnification) Other cells in the culture have no such problem. Can you describe this morphology?

01 January 1970 2,913 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

How can I set up UV irradiation for crosslinking lab?

Hi, I am using a mix of 2 polymers and want to try and crosslink them. I have a UVA lamp of 365nm and have tried the lab previously at 30cm above. How would you recommend I approach the lab and...

31 January 2021 8,711 3 View

How to immobolise streptavidin to polyacrylamide using EDC/NHS?

Hi! We are aiming to bind streptavidin to polyacrylamide surface. The EDC/NHS method bounced on me as a common method, utilizing the -NH2 of polyacrylamide and -COOH of streptavidin. Is there any...

17 December 2020 7,944 2 View

Why HEK293 transfected with LAMP-1GFP do not load dextran?

Hi every one I have made a cell line of HEK293 transfected LAPM-1GFP and after seeding the cells for 24h, I try to load dextran on the dish but after imaging, the cells with GFP+ have loaded less...

14 December 2020 1,801 2 View

How can i figure out whether nanoparticles in my synthesis crosslinked or not?

i prepare protein nanoparticles. in one of my synthesis i use trifle amount of crosslinker and the nanoparticles stick in the bottom of plate. how can i understand that nanoparticles become...

03 December 2020 559 8 View

Does anyone have experience with the Nicoya OpenSPR COOH sensors?

Hi, I am planning on running some protein-protein interaction/kinetics studies using the Nicoya COOH sensors in OpenSPR. I am looking for the activation and blocking buffer composition that are...

01 November 2020 262 1 View

How can my material HMW Hyaluronic acid form a gel within seconds with Eosin Y photoinitiator?

I'm currently working on developing a bioink with HMW HA-TA (hyaluronic acid/ tyramine) and Eosin Y alone. Final concentration of Polymer solution is 2.0 (w/v)% (in PBS) and Eosin Y (in DMSO)...

29 October 2020 8,771 2 View