You place beads of a biomaterial in a bacterial culture expecting the number of germs will increase over time.
How would you proceed to count the number of germs adhering to the surface of the beads without the "noise" caused by microorganisms in the random layer of agar that sticks to a bead?
I suppose you might want to consider a microscopy approach (direct staining and counting with a very high mag scope); or a functional indirect approach might work best (e.g. if you can isolate the beads, do a gentle wash (or not), and perform some CFU count or measure bacterial peptide amount etc. ) ...
For reducing the noise, the washing might work, otherwise if the non-specific binding is expected to be low, perhaps you can simply do baseline controls and subtract them from your "real" results (e.g. place beads on agar and immediately remove after a very short time).... This is a generic tactic usable in many scenarios...
Dear Jackson, Thanks for your suggestions. I will forward this to the our university research microbiology lab to ask them to try to implement these techniques. I hope it will fit within my grant. Best regards, Frederic
Hi Frederic, you could also in ultrsonicator to lyse the cells and measure heir DNA.
Dear Steingrimur, Thanks for your suggestion. I will also submit the approach you suggest to the head of the university microbiology lab. I will also ask her what would be the extra-cost per assay. Best regards, Frederic
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