Dear Maulana,

I had a short time experience of working with earthworms during a project I was assisting after my doctoral graduation and before the spread of covid pandemic. In my opinion with a correct and accurate morphological examination of each individual of earthworm, you can avoid miscalculation. Especially, clitellum is the most important part. First by finding the clitellum; secondly the mouth and anus. But, I should emphatically mention that in juveniles there is the high probability of mistake. In this case I always had to consult with our project head to solve the complexity.

Best, Elaheh

Hello Maulana; What you do might depend on how you were sampling. For instance, Did the sample come from digging out a little plot? If so a cut worm was in the plot...it doesn't matter if the rest of the worm is lost.

You could take Elaheh's advice and count only clitellums. The assumption would be that both parts of cut worms are in the sample.

The dilemma arises out of how the sample was defined before collecting started. Best regards, Jim Des Lauriers

Hi. There are three ways. Kindly, see the following wrebsite to jabe the fill details:

https://www.fwi.co.uk/arable/3-ways-measure-earthworm-numbers

If you count two parts of one earthworm twice as separate earthworms you data and results will not be accurate. I think you will have to recount for accuracy. If it is only one half of the earthworm that is available, you can count it as one but when you have two different pieces indication the head and tail regions, both should be counted as one. I wish you good luck in your research endeavours.

I think gentlemen's answers (Elaheh Daghighi Masouleh's , James Des Lauriers , Aref Wazwaz ,Uzoma Darlington Chima ) convincing you sir Maulana Insanul Kamil. I wish you good luck, success and happiness