I have attached the automatically generated zeta graph (PALS Zeta potential Analyzer. Ver. 5.60)
Please let me know how to convert it to intensity vs apparent zeta potential
If you are performing a PALS measurement, intensity is irrelevant. PALS gives a single, averaged value. It cannot yield a distribution.
In your example, what kind of electric field was applied (i.e., DC or AC)? If it was an AC field, you have to know the frequency. You also need to know the amplitude of the field (usually the voltage divided by the electrode separation), the wavelength of the laser, the scattering angle and, depending on the instrument design, the refractive index of the liquid. You then have to process the raw phase data and fit it to the corresponding equation.
Looking at your example, if you are using an AC field (e.g, 10Hz), then I would interpret your raw phase data as an interfering linear velocity term (e.g., sedimentation or convection).
I'm not familiar with the instrument you used. Who makes it?
I'm the inventor of the PALS technique. My PhD thesis is available (see my publications on RG) which describes the technique. The optical geometry is different to commercial instruments but the mathematics is the same.
Thank you for the response sir.
Actually my interest here is only to know the particle size and Zeta potential and I need to plot the count vs Zeta potential values. It comes as a frequency curve. M not aware of how to do it
The Brookhaven ZetaPALS has offered different combinations of features over the years. At one point, it offered PALS analysis for zeta potential, conventional laser-Doppler electrophoresis for zeta potential, and DLS for particle size. More recently, the instrument called ZetaPALS only offers PALS. I assume you have an older instrument with all three capabilities.
I am not clear what you are trying to do. It sounds like you want to measure the zeta potential and compare it with the raw count rate? Or are you wanting to compare zeta potential with particle size?
Please be more specific with you goal and what you are currently measuring. If you have a PDF of the instrument's manual (or a link to it), I can take a look.
Many years ago, I helped Brookhaven develop the ZetaPALS (the first commercial instrument to incorporate my PhD research - i.e., PALS for electrophoretic mobility/zeta potential).
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