How to show a protein is being dimerized in SDS-PAGE gel?

A protein dimerizes through CTD. I have expressed CTD of protein with two different tags. Now, I want to show that these CTD of protein dimerize in a pull-down assay. But my doubt is that if the...

29 January 2021 7,529 4 View

What are the impacts of pKa1and pKa2 in drug absorption ?

Pregabalin is a crystalline solid with a pKa1 of 4.2 and a pKa2 of 10.6. what insight can i get from this aforementioned information during drug design. What is the difference between pKa1and pKa2.

28 December 2020 7,237 3 View

How to deal with negative factor loadings while performing PCA-MLR to calculate quantitative source contribution in air pollution studies ?

If I am getting some negative values in factor loadings while performing principal component analysis (PCA), then I should use that negative values to calculate daily source contribution using...

22 December 2020 5,248 1 View

What are the source markers of Naphthalene, Acenaphthylene and Acenaphthene in ambient atmosphere ?

Is any article available which reports the sources of these PAHs ?

22 December 2020 8,738 1 View

Why high infiltration in OM treated plot?

We are carrying out trial in the glasshouse where we are comparing the infiltration rate in sodic soil in two different treatments Polyacrylamide vs organic matter ( lucerne pellet). within 27...

25 November 2020 8,787 3 View

Where can one download data (csv, excel) for average daily values of AQI (air quality index) in popular cities, like, Delhi and Mumbai in India?

Air pollution has become a very big problem in Indian cities, especially Delhi. It requires immediate attention and we need to soon understand the trends, provide forecasts for future, issue...

19 November 2020 1,433 3 View

What is the best feasible method to plot DISTANCE vs LST graph?

The above question is intended to be answered in reference to the reduction of the urban heat island by the river.

22 October 2020 7,732 4 View

Which type of measuring instrument can be used to measure hot thermic fluid flow rate?

The flow rate of thermic fluid is less than 1 L/min and temperature ranges from 25 - 200 degree Celsius with a pressure limit of 5 bar.

06 October 2020 5,612 11 View

Can anyone endorse me for arXiv.org?

Hello, I need an endorsement to submit my preprints in arXiv.org. I am in the bioinformatics research field. The arXiv requires an endorsement for me because my institution doesn't provide any...

02 October 2020 1,041 4 View

How to calculate a flow rate over the broad-crest wear for this conditions?

How to find a relationship between velocity and weir landing length (X') as shown in figure?

28 September 2020 7,159 3 View

What is the UV-vis spectroscopy and HPLC UV detector?

I did spectrum scan to levothyroxine sodium which show highest absorption at wave length=230nm which the absorption =0.136 and it's absorption at 225nm =0.047 but when Injecting it on HPLC...

09 February 2021 1,631 3 View

How to determinate phosphatidylcholine content in cow milk?

I would like to determinate the phosphatidylcholine content in cow milk. We only have a Shimadzu Prominence HPLC (High Performance Liquid Chromatography) with a fluorescent detector for the...

18 January 2021 8,370 6 View

Does different HPLC's give different peak areas?

Hello, we recently acquired a shimadzu HPLC and started our analysis through it. What I noticed usually is the peak area of our samples are very different compared to the results got from our...

03 December 2020 610 4 View

Interpreting and troubleshooting anomalous HPLC results?

I am running a simple isocratic Shimadzu HPLC system coupled to a fluorescence detector to detect and quantify biogenic amines from in vivo samples. But my results are not as expected and I'm...

10 November 2020 7,027 5 View

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08 November 2020 7,455 5 View

Why i am getting sharp negative peak while exporting my data not while running? Is this is system problem or column?

I am using Shimadzu HPLC system and recently i started getting sharp negative peak around 5 to 6 min which i was not getting before. I changed the solvent as well to notice if problem is there but...

06 January 2020 4,835 3 View

Why i am getting sharp negative peak while exporting my data not while running? Is this is system problem or column?

I am using Shimadzu HPLC system and recently i started getting sharp negative peak around 5 to 6 min which i was not getting before. I changed the solvent as well to notice if problem is there but...

31 December 2019 2,556 3 View

For saturated hydrocarbons in the extracts, why only n-alkanes exist but steranes and terpenes are absent ?

All Ordovician limestones were extracted to get the extractions, and the saturated hydrocarbon of which had been analyzed using a SHIMADZU GC-2010Plus equipped with an FID. The results showed that...

11 December 2019 7,960 2 View

Sometimes while performing uv vis of thin films I used to get transmission more than 100% . How to resolve this issue?

I am keeping the lamp warm for atleast 30 min but still I m getting 110% transmission . I am using Shimadzu solidspec-3700 uv- vis-nir spectrophotometer. Please help to resolve this issue.

11 December 2019 8,242 9 View

I'm having a hard time to get the UV-vis spectra of acetone. What may be the reason?

I'm getting a weird UV spectra for pure acetone. The analysis were performed on Shimadzu UV-vis spectrophotometer however the graphs weren't good with noise patterns as seen on the image. Any help...

11 November 2019 9,477 5 View