Greetings, good people,
I kindly request assistance in converting concentrations from µg/ml to µM.
While calculating the concentrations to evaluate compounds that may inhibit cathepsin S (the enzyme of interest) and assess their cytotoxicity using the MTT assay, I used units in "µg/ml." Now, I intend to convert them to µM, as most previous studies have utilized this unit.
The compounds in question are chlorogenic acid, quercetin, and procyanidin. According to the literature, the conversion is given by the formula: µM = µg/ml ÷ molecular weight (kD).
Regarding the conversion of g/mol to kD, we said 1 kD (kiloDalton) is equivalent to 1,000 g/mol.
For chlorogenic acid, with a molecular weight of 354.31 g/mol. Is it correct to say 10 µg/ml is equivalent to 28.22 µM?
This comes from this calculation.
µM = µg/ml ÷ (molecular weight in g/mol ÷ 1,000)
= 10 µg/ml ÷ (354.31 g/mol ÷ 1,000)
= 28.22 µM
11/23/23
Dear Sanele,
The formula you have and your calculations are correct.
Good luck w/ your research.
Bill Colonna
Dept. Food Science & Human Nutrition
Iowa State University, Ames, Iowa, USA
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