Does Anyone Know of a Way to Detect Melatonin that is NOT HPLC, RIA or Mass Spec?

I tried to do a Slot Blot and was not successful and melatonin is only .23 Kda so it would probably run off a western blot. Has anyone had success with other methods?

23 January 2021 1,388 3 View

Any advice or articles on combining one qualitative approach with the methods of a different approach?

I am a doctoral student and I am looking to do a qualitative research study that looks at the potential influence of teachers on the way minority students view their leadership capabilities....

28 November 2020 6,217 5 View

Troubles cloning - correct RE digestion and then incorrect sequencing?

I am inserting an MPP5 gene insert into a pCAGGs backbone. When I am finished with the mini prep, I find that my RE digestion is correct, but then when I send it to be sequenced, the MPP5 gene is...

23 November 2020 7,962 2 View

What are the available Nanocomposite Databases?

I am looking for data files of Nano Composites. Any form of data, be it XRD outputs, SEM or TEM images or anything. Where can I find them?

28 August 2020 9,927 5 View

Can I degas drug solutions before applying to mammalian cells for electrophysiology?

I am applying different drug solutions to cells that I am whole-cell patching. Each solution is in a syringe connected to a thin tube that goes through a barrel manifold into a glass micropipette...

24 August 2020 1,956 2 View

How to count % GFP positive cells and GFP intensity using imagej?

I have infected BHK21 cells with a virus that expresses GFP. I want to count the percentage of GFP positive cells and the intensity of the GFP. How do I do this with fluorescent microscope...

17 August 2020 4,009 2 View

CD8+ T cell and natural killer isolation kits from rat spleen?

Hello, Does anyone have any experience with isolation of CD8+ cytotoxic T cells and natural killer cells from rat spleen? I'd prefer kit-based if possible, but any protocols you can provide would...

30 July 2020 5,387 6 View

Best ways to analyze crowdsourced data for a qualitative study?

Hello, I am currently working on a project analyzing crowdsourced data from online trip reports and social media applications. However, I am struggling in defining the methodology for this...

25 February 2020 4,401 3 View

Porcine specific antibody that doesn't react with rabbit?

Does anyone know of an antibody that can be used for immunohistochemistry on paraffin embedded sections that reacts with porcine cells but not rabbit cells? We need to prove that the porcine cells...

20 January 2020 9,014 2 View

Which statistical test should I use?

I am using Minitab to analyse my results. I have three treatment conditions (A/B/C) and 10 subjects (the same subjects do all three conditions) and for each condition, I am recording the time...

19 January 2020 1,123 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View