Hi,

I am studying the effect of a particular compound on a bacterial pathogen. By treating the compound with the bacterial culture, I found that the CFU is reducing. Then, I checked the growth rate of the bacteria in the presence/absence of the compound by measuring the OD at regular intervals of time. Here I found that the compound slows down the growth rate (long lag phase, short exponential phase, and reduced stationary phase).

How do I confirm the compound impacts the growth rate only or it has a killing effect? Any experimental inputs or suggestions would be helpful.

Thank you.