Is there any simple chemical analysis method, by which the removal of outer sericin protein layer from raw silk fibers (after de-gumming) can be confirmed?
There are techniques likes weight loss, FTIR, FE-SEM, XRD, SAXS, Contact Angle, DSC, & TGA, for the confirmation (I have tried these techniques).
But, I want to know about any simple chemical analysis method, which can help in confirming the removal of sericin protein layer from de-gummed silk.
Thank you.
Kind regards,
Prakash
https://www.hindawi.com/journals/bmri/2016/8175701/
https://pdfs.semanticscholar.org/25be/8815f6e21626d65b334d822466adf4f36bae.pdf
The quite universal method is to check the weight loss (should be between 25 and 30%), then image analysis can help (SEM). Spectroscopic methods would be inefficient because both fibroin and sericin are proteins and they are difficult to separate in FTIR.
GPC could be a nice idea, cause the the two molecular weight are quite different.
Hi Alessio Bucciarelli ,
Thanks for the valuable information.
It is helpful.
Kind regards,
Prakash@
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