How to condition a new C18 column? What are the prerequisites and cautions? What are the solvent concentrations you suggest?
Check https://www.nacalai.co.jp/global/cosmosil/pdf/Technical_Notes8.pdf
https://vneshbiotorg.ru/upload/welch/use-and-maintainance-of-hplc-column-welch%20materials.pdf
Also check https://www.chemass.si/the-cleaning-and-regeneration-of-reversed-phase-hplc-columns/
https://www.youtube.com/watch?v=JYaLzRUxI7s&vl=en
Also check on this paper
https://www.mdpi.com/2227-9717/10/2/259/pdf
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