How to comply ddCt qPCR data with Adding the dots to the Plots ?

08 August 2018 0 9K Report

Nature Biomedical Engineering recently published this article regarding showing the actual data points on bargraphs in order for the data to speak for themselves.

https://www.nature.com/articles/s41551-017-0079

The data used in the article can be downloaded from

https://figshare.com/articles/May_2017_Editorial_Nature_Biomedical_Engineering/4928888

However, I am very struggling with how would one generate ddCt data from a triplicated RT-qPCR experiment.

How would one generate points in the first place?

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