Hi Everyone, I am doing a PK study on mice by injecting a drug intranasally. I need to collect nasal tissue. Has anyone done this before? Thanks
Good day, Radwa Soliman . You can do make sectioning of a whole head as described in COMPARATIVE ANATOMY AND HISTOLOGY
A MOUSE, RAT, AND HUMAN ATLAS/the second edition (Editors: Piper M. Treuting et al) 2018 at page 46
Hello, I am looking for a pdf of this paper. Thanks for help! Benedek PN, Sarjeant W. 1981. Dinoflagellate cysts from the Middle and Upper Oligocene of Tonisberg (Niederrheingebiet): a...
16 July 2024 8,683 0 View
I have data for a cross-over randomized controlled trial. I wonder if I can use MANCOVA to compare my outcomes (they are continuous) while having paired data. If I can't, what would be an...
20 November 2023 9,584 4 View
Can someone share their protocol for culture of primary human adrenal cortical cells please. Thank you in advance!
15 October 2023 6,971 0 View
I have run a multiple imputation model and after it I used the with() and pool() functions to pool all my results using linear regression to get one estimate. I want to run mediation analysis on...
13 October 2023 9,124 1 View
I need to calculate sample size based on this equation but I am give hedges g instead of cohen D: pwr.t.test(d = , sig.level= , power = , type = c("two.sample")) • d=effect size •...
31 August 2023 572 3 View
Hello there, I'm searching for reliable bioinformatics/immunoinformatics tools for predicting the immunogenicity of B-Cell Epitopes. Your expertise is invaluable! Could you kindly recommend any...
17 August 2023 1,725 1 View
Hello, I recently stumbled on a paper in the field of neurosurgery. The paper analyzes imaging parameters that predict injury related to the spinal cord (Posterior Ligamentous Complex Injuries)....
17 May 2023 7,382 1 View
Hello, We are currently conducting a network meta-analysis of RCTs using R (netmeta package). I have relatively small number of studies. The problem is interventions that are non-significant but...
30 April 2023 9,977 1 View
I am trying to establish a dose-response relationship with different concentrations of LPS on differentiated THP-1 cell-secreted TNFa and IL-1b. However, I am finding that all the concentrations I...
08 March 2023 4,316 1 View
I am so interested in this reference, can you help!! Kirsch, K.-H., 1991. Dinoflagellatenzysten aus der Oberkreide des Helvetikums und Nordultrahelvetikums von Oberbayern. Münchner...
27 February 2023 1,068 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View