if possible please share the procedure ?
This experiments need rats or microorganisms to know LD50
You need a toxicity protocol which contain gradually plant extract dose
Check https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-017-2005-8
Also check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141048/
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