if a cell leading death while treating with compound X .want to study redox change in cell how can measure redox homeostatsis?
Dear Pooja!
According to your question the cell dies on treating with compound X. I suppose the compound X is redox active compound ( this you have also mentioned in your questions) most probably the compound X brings in the high level of oxidative stress in the cell on account of which the cell collapses. If this is so then to check the redox change in cell we can use the methods of quantifying the oxidative stress in cell and for this we have many protocols depending upon the type of ROS species generated in the cell for the build up of oxidative stresssome of these can be:
1.ROS-Glo™ H2O2 Assay: A rapid, sensitive bioluminescent assay to identify conditions or test compounds that alter ROS levels. Eliminates false-hits associated with HRP methods.
2. GSH-Glo™ Glutathione Assay: Detects and quantifies glutathione in cells or in a variety of biological samples, providing a sensitive alternative to colorimetric and fluorescent methods. Suitable for HTS.
3. GSH/GSSG-Glo™ Assay:Detects and quantitates total glutathione and GSSG to allow calculation of ratio of reduced (GSH) to oxidized glutathione (GSSG).
see link https://www.promega.in/products/cell-health-and-metabolism/oxidative-stress-assays/ros_glo-h2o2-assay.
Dear Pooja!
According to your question the cell dies on treating with compound X. I suppose the compound X is redox active compound ( this you have also mentioned in your questions) most probably the compound X brings in the high level of oxidative stress in the cell on account of which the cell collapses. If this is so then to check the redox change in cell we can use the methods of quantifying the oxidative stress in cell and for this we have many protocols depending upon the type of ROS species generated in the cell for the build up of oxidative stresssome of these can be:
1.ROS-Glo™ H2O2 Assay: A rapid, sensitive bioluminescent assay to identify conditions or test compounds that alter ROS levels. Eliminates false-hits associated with HRP methods.
2. GSH-Glo™ Glutathione Assay: Detects and quantifies glutathione in cells or in a variety of biological samples, providing a sensitive alternative to colorimetric and fluorescent methods. Suitable for HTS.
3. GSH/GSSG-Glo™ Assay:Detects and quantitates total glutathione and GSSG to allow calculation of ratio of reduced (GSH) to oxidized glutathione (GSSG).
see link https://www.promega.in/products/cell-health-and-metabolism/oxidative-stress-assays/ros_glo-h2o2-assay.
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