How to check pH sensitivity of a peptide?

19 April 2025 1 6K Report

What are the studies need to be taken to confirm the pH sensitivity of randomly selected peptide sequences.

To check pH sensitivity of a peptide, analyze its sequence for ionizable residues and experimentally assess structural or functional changes across different pH values using spectroscopy and activity assays. Confirm pH-dependent behavior by determining pKa values and comparing with controls.

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