I am trying to measure a biologic's effect on proliferation of a cell line. The cells secrete a stimulant to induce proliferation so I don't need to add the stimulant as a separate treatment.
I have blank wells (no cells), negative control wells (cells without the inhibitory reagent), and I want to measure proliferation after 5-7 days so I also have cells in a different plate to be measured on Day 0 to establish the baseline for proliferation.
How should I calculate % cell viability?
I can use this formula:
percentage of cell viability = (Atreatment − Ablank)/(Acontrol − Ablank) × 100%
But is there a way I should add my Day 0 data to the calculation?
Thank you.
I think you don't need to add day 0 to your calculation. The day 0 data just tell you that you seeded the same numbers of cells in your plate. If the day 0 cell viability are the same among different samples, you don't need to normalize your data. If not, you need to normalize your day5-7 data to day 0.