Helen Wong

5 Questions 1 Answers 0 Followers

Questions related from Helen Wong

What is the best way to isolate PBMC from whole blood for freezing?

What is the best way to isolate PBMC from blood for freezing? RBC lysis method with ammonium chloride or density gradient with Ficoll? Has anyone done comparison of both methods with cells that...

19 July 2022 3,859 3 View

How to calculate % viability in anti-proliferation assay and use Day 0 data as a baseline?

I am trying to measure a biologic's effect on proliferation of a cell line. The cells secrete a stimulant to induce proliferation so I don't need to add the stimulant as a separate treatment. I...

03 October 2019 717 1 View

How can I get rid of air bubbles trapped under coverslip for 8 chamber slide?

We culture cells in 8 chamber slides and do immunofluorescent staining. We put one drop of mounting media on each of the 8 wells and drop the coverslip at a 45 degree angle. Does anyone have tips...

19 January 2016 8,931 4 View

How is glucose concentration in beta-cell (islet) culture chosen?

I'm wondering why we normally use 11.1mM (human) and 16.5mM (mouse) glucose in islet culture.

09 December 2015 4,050 3 View

Intrinsic apoptosis: what happens to cytochrome c after it's released and forms the apoptosome?

Does released cytochrome get degraded after it binds to apaf-1 and does it form the apoptosome? I also heard from my supervisor that it goes back to the mitochondrial membrane, which seems...

03 December 2014 9,661 3 View