31 August 2019 1 8K Report

For laterally loaded piles subjected to axial and bending forces, how to calculate the pile capacity for moment and shear??

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Inquire about adding a reviewer?

Dear Researcher Gate, I inquired about adding a reviewer to the submitted manuscript in Biochemistry can you help me with this? Thanks maha saad

07 March 2024 326 2 View

Chip-seq of scraped colonic epithelial cells?

Hello everyone, I would like to know how to purify the scraped colonic epithelial cells from the non-epithelial cells. P.S I could not use flow Cytometry as it will take time and I need to fix...

12 February 2024 6,706 0 View

How to conduct a pre-whitening process to the time series data using Excel?

I am working on a project that involves monthly time series climate data for 33 years. I want to use the Mann-Kendall test to examine the trend of the variables. However, I encountered a problem...

04 January 2024 6,721 0 View

Are there any ongoing collaborations within the realm of fixed point theory?

I am currently researching various contractions using a measure of noncompactness for both single and multivalued mappings. My study focuses on exploring the existence of solutions for equations...

29 October 2023 4,902 3 View

What should I do if too light band get on 3% agarose gel after overnight incubation in RFLP?

The PCR product that I used shows 170 bp product in a separate gel. But also contains its recognition site according to the reference paper. I used the protocol as provided by the Thermoscientific...

25 September 2023 3,727 2 View

False negaive mediastinal LN in patient with cN1 NSCLC?

I am working on a specific study and I think it is unique because I want to minimize false negative staging in mediastinal LN, so what has been conducted was using the Pathology report and took...

04 September 2023 2,604 1 View

What is support vector machine algorithm?

what is a support vector machine algorithm?

10 July 2023 3,438 8 View

How ageing can be delayed; what is pathophysiology and its latest research protocols?

How ageing can be delayed; what is pathophysiology and its latest research protocols?

22 May 2023 9,744 1 View

About epithelial culture?

I used Millicell-24 Cell Culture Plate, fixed trans-well PCF for colonic epithelial monolayer. the PCF Filter with coated Matrigel including the cells. the filter is translucent and I couldn't...

02 April 2023 696 0 View

What solvent can we use to wash LiPF6 salt from electrodes as anode for Lithium-ion battery (LIB)?

Please, give me some choices. Thank you.

04 January 2023 3,667 2 View

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How to make a Lateral flow control line more uniform?

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Do you agree that at some point in our lives each of us will be a person with disabilities?

There is several approaches towards the analysis of legal status of people with disabilities (e.g. medical, social or human rights approach). One way to include people with disabilities in society...

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What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized?

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...

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Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

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Hlw, I am trying to build a model a single pile in 3 layers soil in Flac-3D. Could you please help me about the Flac-3D code?

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Why liposome size increase during storage?

My liposome is a non-phospholipid liposome (sterosome) composed of palmitic acid and cholesterol, 20mg of palmitic acid and 70mg of cholesterol. After several months of storage in a refrigerator...

18 June 2024 7,459 5 View

How can i use hexamethyldisilazane(HMDS) for drying instead of Co2 and freez drying for SEM analysis of rice anther and pollen?

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Can HCl be used instead of H2SO4 for RNA later preparation?

I am preparing RNA later following the patented protocol. It is mentioned there and in other recipes as well to use H2SO4 to balance the pH to 5.2. If H2SO4 is not available, can I use HCL instead...

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Cell culture contamination?

Hello everyone, Recently, I have encountered a problem in cell culture. I use Rpmi 1640. I have freezed SKBR-3 cells 8 months ago. I am defreezing them now. I defreezed two cryovials up to now and...

22 May 2024 8,278 4 View

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