Fluorescent tyramide protocol ?

Hello, Im asking to check if anyone has a protocol for preparing fluorescent tyramide for fluorescent in situ hybridization. The one Im using isnt working, so if anyone has one that works, can you...

05 November 2020 9,566 3 View

How to analyse hydrophobic organic cpds in animal fat tissue?

Am looking for the best method to extract and analyse water insoluble organic cpd from animal fat tissue

12 October 2020 1,586 2 View

How do I run a western blot following co-ip?

This is the first time I do any sort of IP experiment and I was wondering if there is a certain procedure to follow for the western blot after the last elution step in the co-ip protocol. I am...

22 September 2020 3,688 3 View

Does anyone have a copy of this article?

Bartlett H, Westcott L, Hind P, Taylor H (1998) An Evaluation of Pre-Registration Nursing Education: A Literature Review and Comparative Study of Graduate Outcomes. Oxford Centre for Health Care...

07 September 2020 552 14 View

Nanotechnology question. Getting rid of solvent after LNP formulation?

I'm dealing with really micro volumes and I'm trying to figure out if there are other efficient methods aside from dialysis to get rid of residual solvent for LNP purification. And if not, are...

25 May 2020 6,377 1 View

Unbalanced data in linear and nonlinear mixed model ?

when fitting a nlme on nested repeated measurements data , models can be unstable if sample sizes across groups are highly unbalanced. In my dataset I have 4 unbalanced groups in terms of...

24 June 2019 1,698 4 View

Is soxhlet extraction a reliable way of getting pure THC from cannabis plant?

I have been having isssues getting pure THC form the USA and it seems the price from sigma is exhorbitant. I want to get the plant and extract THC. i was told by someone that soxhlet extraction...

15 August 2018 8,300 9 View

Can someone recommend how to get the full ATDP scale by Yuker & Block ?

I am looking for how to get the ATDP scale for a study and I'm wondering if it is copyrighted and who to contact in order to get the scale.

28 October 2016 2,295 1 View

How can I analyse data that is not mutually exclusive?

I have a set of data whereby one of the questions states: How many foods from the following list do you eat on a weekly basis. Tick all that apply. There are 8 possible answer options which...

03 March 2016 4,792 3 View

How can i adjust parameters in logistic regression?

26 February 2016 1,440 13 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View