We are trying to investigate the sorption desorption of pesticides in soil. We are following OECD 2002 Standards for this. After completion of the sorption study, we are replacing the supernatant with a pesticide-free solution to check its desorption. But the equilibrium concentration shows more pesticides than the amount adsorbed in soil. so we are getting a negative desorption value. is one cycle of desorption study enough or do we need to do more than one to get the desired result? also how to calculate the desorption.

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