Stability constants attached.
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Currently I am studying the absorption behaviour of multilayer thin film sample using XRF.The thin film has 3 layers ( WC/Co as substrate, Ti(C,N) as middle layer and Al2O3 as top layer). Here...
26 June 2024 6,065 2 View
Baumeister
16 March 2024 4,525 2 View
Hi.. I want to do least cost path and corridor analysis from genetic data. I want to show possible dispersal corridor using hapotype (genetic data) for my target species. I know it can be done in...
12 March 2024 4,505 3 View
Dear Concern, Hope you are doing well. I am conducting a research study on the *“Knowledge and Use of ICF for health needs assessment and rehabilitation: A Survey in Pakistan”* to investigate...
07 March 2024 367 0 View
I wanted to explore the interaction between A and A-SYN. In the first two experiments, I set up three experimental groups with three parallel samples in each group according to the concentration...
12 September 2023 4,449 0 View
How to design qPCR primers to detect the constant regions of Ig
27 July 2023 8,754 1 View
I understand the extreme pH can kill the normal skin microflora bacteria. But how. can someone explain the process?
21 February 2023 10,049 4 View
When analyzing my PCR results, I found apart from amplification of my expected band I also another unusually amplified band at 250 bp from both my control and recombinant strains. I believe that...
22 April 2022 9,213 3 View
I was using fluorescent dye PET with a SSR primer and the fragment peak was at 180 and 184 (in first set of 48 accessions), and now i am using dye SUN with same primer used for PET, giving the...
25 February 2022 2,978 0 View
How to prepare the input file for software PERMUT to calculate Gst and Nst. I have 14 haplotypes from 15 populations (6-8 individuals in each population) and i need to know the presence of...
14 September 2021 3,916 0 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
I understand that there are differences in ion preference as far as chelation is concerned when comparing EGTA vs EDTA. But is it ok to replace EGTA in my lysis buffer with EDTA since EDTA...
10 June 2024 1,188 1 View
My buffer components consists of 150mM Nacl 5mM HEPES 20mM EDTA and volume is made up to 10ml by ultrapure water what is the osmolarity of this soln ?
16 May 2024 5,245 0 View
Hi everyone, I've been trying to replicate the protocol described in this paper: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22736012/ for separating aminoacyl-tRNAs from deacylated tRNAs...
14 May 2024 7,257 3 View
I am isolating the crypt from mouse small intestine the following several modification of EDTA concentration, 3mM (30 min), 5mM (30 min), 20mM (25 min) at 4 degree. For all the EDTA...
09 May 2024 9,005 2 View
I had problems in obtaining a good CD45 positive cell population after digestion with Liberase. I used same Liberase based on published protocol, the digestion time of 50 mins,37C, and similar...
03 April 2024 8,921 0 View
