Can we use RNA-seq library for gene expression studies?

Since our RNA is less abundant, can we use the libraries prepared for RNA-seq to validate (qPCR)the results of RNA-seq.

12 November 2020 803 6 View

For primer efficiency calculation can we use positive control(in which gene is abundantly expressed) and perform the analysis?

My sample of interest is sperm RNA(less abundant and highly fragmented RNA). In that case, to determine the primer efficiency, can i use tissue samples (in which the gene is abundantly expressed)...

11 November 2020 758 3 View

What is the difference between semantic role labelling and named entity recognition?

Natural language understanding

04 May 2019 7,442 9 View

Acyliminium reactivity versus enecarbamate?

Dear researchers, i kindly ask you to help me with the following aspect: in scheme 4 (of j.org. chem, vol.66,No.23, 2001) it is shown that nucleophile attacks on the acyliminium intermediate and...

08 February 2016 1,601 3 View

I am using HEC RAS to connect various ponds and lakes in the city and perform flood inundation mapping. What is the suitable analysis?

I am using HEC RAS (Though it is a river analysis software) to an urban area to connect various ponds and lakes in the city and perform flood inundation mapping. What is the suitable...

01 February 2016 4,321 6 View

Why does K estimator fail to estimate Ka and Ks values for certain gene combinations?

12 February 2015 8,709 1 View

Can any one explain me the pixel recursion and how to calculate new disparity candidate using this?

Pixel recursion.

11 August 2014 2,495 2 View

Can anyone guide me about disparity estimation in stereo images using line-wise hybrid recursive matching algorithm?

Disparity estimation of stereo images. This involves several processing steps including pixel recursion, post processing, how to estimate disparity between two gray scale images using this algorithm.

11 August 2014 9,312 1 View

Why is a logarithmic current amplifier used instead of a linear one in scanning spreading resistance microscopy?

For measuring and amplifying current during a C-AFM scan, I'm aware that a linear current amplifier is generally used but in SSRM which is mostly used to get doping profiles in semiconductors, a...

11 April 2014 4,026 3 View

What is the exact definition for entropy or what does entropy really mean?

Entropy is defined relatively with temperature and enthalpy of the system but what type of characteristic is entropy?

20 February 2014 5,501 9 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View