What is the difference between filler and fiber?

Explain in correct manner

28 December 2020 5,199 2 View

I have a list of properties of tool steels here. Please let me know which of the below mentioned properties will change after heat treatment?

16 August 2020 730 8 View

Implementation of Langevin Dynamics in Fortran?

I am trying to implement a FORTRAN code that can perform NVT simulation using Langevin Dynamics. I have been following the textbook by Allen and Tillesdly for the initial implementation of the...

17 April 2020 5,309 3 View

How to insert Magnetic B-H Curves in Ansys Mechanical?

25 March 2020 7,998 3 View

How to calculate the current (in amperes) flowing through a volume, when a current density is applied on the volume ?

Hello, I would like to know how to deduce the amount of current flowing through a solid volume , when current is applied in the form of current density 'J' (A/m2). Example: As shown in the image...

21 October 2019 8,306 6 View

Has anyone tried the RTF optical tissue clearance technique?

I wanted to know if anyone has any experience with this rapid tissue clearing technique, link to the paper - https://www.nature.com/articles/s41598-018-20306-3 It looks very promising, but I am...

02 May 2018 9,014 4 View

What is the Procedure for Soxhlet extraction for isolation of beta sitosterol from plant material?

Can anyone suggest a procedure for soxhlet extraction for isolation of beta sitosterol from dried plant material. which is the best solvent to use? Now I am using hexane as solvent.

02 April 2016 8,130 4 View

How do I reduce the loading time of protein on chromatography columns?

I'm trying to reduce the loading time of desired protein from clarified harvest on affinity columns, but I'm not getting it. We should select the resin based on the binding capacity and also...

02 October 2014 5,773 9 View

How to compute the rate of heat release in an gasoline engine?

The mathematical formulae to compute the rate of heat release? how can it be related to exhaust temperature?

01 January 1970 2,297 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Why cloning of gene into pCAMBIA1304 vector is unsuccessful?

I have tried to clone a specific gene into pCAMBIA1304 vector. But all the attempts failed. I used ligation kit from both Promega Corporation and Takara Bio. However, there was one time I used...

04 February 2021 525 1 View