We know to calculate DW factor as S = − ln ( Intensity(ZPL) / Intensity(total) ) , but I want to find the HR factor similarly , how can I do that ?
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
HI , i am concerned about any resist hardening effect of Post bake my LOR +PPR coated sample @90C for 5 mins. I wanted to do liftoff in acetone but the metals are hard to peel off. I cant use PG...
18 June 2024 5,741 0 View
Can we restrict the vertical and horizontal displacement in two directions for the lower semicircular in bottom hole of CT specimen and apply incremental displacement in the upper semicircular in...
09 May 2024 5,272 4 View
It is very well known that, owing to the soft lattice, and ionic bonds, metal halide perovskites shows easy movements of different ionic species under external perturbation. Due to this the...
28 March 2024 4,924 2 View
As we know, lymph nodes have a mixed population of cells, how do we ensure only B cells are getting isolated and subsequently fused with myeloma cells? Thanks in advance!
30 January 2024 3,449 2 View
What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes withou using ready to use lymphocytes seperating medium?
29 January 2024 3,221 0 View
Is Low melting point agarose and agarose having low EEO the same? Is it possible to utilize agarose low EEO instead of low melting point agarose for COMET assay?
29 January 2024 6,834 2 View
have simulated a hydraulic jump. There is a difference in water and ambient(air)temperature and as such there is heat transfer across the water-air interface. I have used VOF model and Standard...
17 September 2023 5,276 1 View
Why do ExpiCHO cells need 8% CO2 levels in the incubator? Is is because they have high metabolic rate, thereby producing more lactate and needing more CO2 to buffer the media? Would like to know...
26 August 2023 6,707 3 View
I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...
30 July 2024 8,770 3 View
Dear all, I have noticed, as the ZnO content increases, the Raman intensity at 100cm-1 also increases. However, there is a peak nearby at 130 cm-1, which I could not find in any paper related to...
28 July 2024 5,409 1 View
These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"
17 July 2024 6,518 3 View
Dear all, I'm currently working with CHI potentiostat and performing Impedance. However, for the circuit fitting the instrument must be connected to the computer. Since I prefer to analyse the...
10 July 2024 8,880 4 View
I have to compare the FTIR of the two samples, one is in powder form and other one is the powder dispersed in IPA or water. Both are same material, so how the bond will change
09 July 2024 8,011 2 View
I'm currently analyzing the Raman spectra of coloured plastic samples and have encountered a wavy, oscillating pattern in the spectra of some coloured samples. I was wondering what the possible...
04 July 2024 1,947 4 View
Hello, I am having a difficult time finding the incident plane wave wavelength dependent spectra of the lights source to find the EQE. Please advise.
26 June 2024 7,274 1 View
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22 June 2024 6,199 0 View
For smoothening of noisy CD spectra, several fitting models are available in Jasco spectra manager software, like Savitzky Golay, binomial, means movement and adaptive smoothening. Each one asks...
16 June 2024 3,997 1 View
Is there any specific method to calculate raman spectra using vasp?
10 June 2024 871 1 View