11 December 2017 1 2K Report

Dear scientists, I need your favor, I used Sergiev et al. [Sergiev et al, 1997 protocol for H2O2 assay. Leaf tissues (500 mg) were homogenized in ice bath with 5 ml 0.1% (w/v) TCA. The homogenate was centrifuged at 12000×g for 15 min and 0.5 ml of the supernatant was added to 0.5 ml 10 mM potassium phosphate buffer (pH 7.0) and 1 ml 1 M KI. The absorbency of supernatant was read at 390 nm. The content of H2O2 was obtained by a standard curve (given below). Now how to calculate H2O2 content on fresh weight basis, please help me

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