I want to calculate the E:S ratio of a dormant seed. After 24 h of imbibition, each seed was cut open along the longitudinal axis with a razor blade, and embryo length was measured.
Now I have to measure whole seed length or endosperm length. Please tell.
this article may be helpful to you.Article Intermediate complex morphophysiological dormancy in seeds o...
U can use a light microscope or electron microscope for seed embryo measurement.
Thank you sir for your valuable suggestions.
Sir whole seed length should be considered for measurement or only endosperm length should be measured Pravin Rawat
In case of samara remove wings first then measure seed length and embryo length. Further, embryo length/seed length will be the ratio@Akshit Kukreti.
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