How to measure fluorescence intensity of Dapi stained nuclei?

I have tried various methods using imageJ to measure mean fluorescence intensity of dapi stained nuclei but none of them seem to be working. Ive attached an image i am trying to measure the...

15 February 2021 8,637 3 View

Dflux and urdfil subroutine input in abaqus?

I am using coupled transient thermal electric step to simulate thermal and electric flux load on cfrp layered composite. DFLUX been used for surface heat flux and URDFIL for element deletion but...

25 December 2020 2,551 1 View

How stiffness matrix modification after hinge formation is done for pushover analysis?

Can anyone please explain this? How do I account for plastic hinges in my model and how do I modify the stiffness after plastic hinge is formed? Need a small example. Thank You

15 November 2020 1,967 3 View

How to model hysteresis property of concrete in abaqus?

I want to model rc element subjected to cyclic loading, How do I enter hysteresis data in the material model? I want to use Mander's 1988 model to get data for concrete. I dont know how to enter...

06 September 2020 7,651 3 View

How to select appropriate value of dispersion coefficient for HecRas 1-D steady water quality model?

I am working on a water quality model for the Mula Mutha river. I have a doubt regarding the dispersion coefficient to be entered. whether to enter a fixed value or computed value? what are...

22 May 2020 848 1 View

Why the ZDOCK output file (.pdb) is not compatible for Chimera?

Why the final docked complex file output from ZDOCK is not compatible for Chimera?

24 April 2020 3,347 3 View

Got frequency of human recorded audio file in the range of 0-8000 Hz. Is it correct range?as per many sources human voice frequency is below 260 Hz?

I tried finding frequency by various tools such as audacity (got frequency 0-16000 Hz). Tried with Librosa(Python package) and got the frequency of human recorded audio files in the range of...

06 April 2020 7,607 6 View

Is there any software which take input as pdf(research papers) and generate the data from all the plots and graph inside it?(Similar to NLP)?

I am looking for software that will take input as a research paper (pdf format) and generate the data from plots and graph in it. as similar to NLP softwares.

04 February 2020 4,244 3 View

How to increase RNA yield and purity from PBMCs stored in RNA later at -80 C?

I have been using miRNA Isolation mini kit (qiagen 217004) for isolating RNA from PBMCs stored in RNA later at -80 C. The cell no. was 2 million cells. The RNA yields obtained are around 30-40...

15 October 2019 4,731 1 View

What is the criteria to select the range of normal stress in Direct shear test?

I did not not find the answer anywhere. Please help.

19 August 2019 9,950 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View