Hi, I have results of q-RTPCR for Control and Experimental groups. I know how calculate the fold change for Experimental group. I obtained results, there are no differences between Control and Experimental groups. However, I calculated the individual Fold changes separately for each Experiment group sample by using general mean Ct values ( including the biological and technical replications ) of Control group. I saw that fold changes were increased or decreased for some samples.
I want to ask how I analyze that these fold changes are significant or not statisticaly?